Journal: Journal of Nanobiotechnology

Article Title: Visualization of the process of a nanocarrier-mediated gene delivery: stabilization, endocytosis and endosomal escape of genes for intracellular spreading

doi: 10.1186/s12951-022-01336-6

Figure Lengend Snippet: Pharmacological inhibitor blocks the SPc-mediated dsRNA delivery. a , b’ Inhibitor application hindered the cellular uptake of ds eGFP /SPc complex. The cells were exposed to 0.2 μM Bafilomycin-A (inhibitor) for 30 min, incubated with ds eGFP /SPc complex, and then examined. Blue: DAPI. Green: ds eGFP . c Schematic diagram of pharmacological assay toward S. frugiperda . The 2-day-old larvae were fed with Baf A for 2 days. Then, RNAi was performed using 4-d-old larvae. d QRT-PCR analysis of ATP-d gene expression in larvae fed with inhibitor. Each treatment consisted of 3 replications. Different letters on columns indicate significant differences (Tukey HSD test, P < 0.05)

Article Snippet: For in vitro test, the cells were exposed to 0.2 μM Baf A (MCE, USA) for 30 min, treated with fluorescent ds eGFP /SPc complex, deposited on the slides using an Antifade Mounting Medium with DAPI, and then examined using a confocal microscope.

Techniques: Incubation, Quantitative RT-PCR, Gene Expression